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il 10 secretion  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec il 10 secretion
    Il 10 Secretion, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+10+secretion+assay+cell+enrichment/IL-10+Secretion+Assay+-+Cell+Enrichment+and+Detection+Kit+(PE)%2C+human/pm39097919-38-8-22
    Average 92 stars, based on 3 article reviews
    il 10 secretion - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Isolation:

    Article Title: Repetitive Antigen Responses of LDL-Reactive CD4+ T Cells Induce Tr1 Cell–Mediated Immune Tolerance
    Article Snippet: Human peripheral blood mononuclear cells were isolated from heparinized blood of healthy donors by density gradient centrifugation (Histopaque-1077) and stimulated with or without 10 μg/mL human LDL (MyBioSource) for 16 hours. .. LDL-specific IL-10 secreting cells were isolated using IL-10 Secretion Assay–Cell Enrichment and Detection Kit (Miltenyi) according to the manufacturer’s instructions. ..

    Article Title: Treating activated regulatory T cells with pramipexole protects human dopaminergic neurons from 6‐OHDA‐induced degeneration
    Article Snippet: The cells were stimulated with anti‐CD3/CD28 ImmunoCultTM Human CD3/CD28 T Cell Activator (StemCell Technologies) for 4 days, and then treated for 24 h with pramipexole at concentrations of 2 and 200 ng/mL. .. Responder cells were labeled and isolated according to IL‐10 secretion using the IL‐10 secretion assay Cell Enrichment and Detection Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: Interleukin-10 + Regulatory B Cells Arise Within Antigen-Experienced CD40 + B Cells to Maintain Tolerance to Islet Autoantigens
    Article Snippet: .. IL-10-producing B cells were isolated using the IL-10 Secretion Assay - Cell Enrichment and Detection Kit Microbeads (Miltenyi Biotec). .. 2x106 PBMC were cultured in the presence of GAD335-52 (100 μg/ml), ProinsulinC19-A3 (100 μg/ml) peptides (Thermo Fisher Scientific, Waltham) with or without isolated IL-10-producing B cells in a 48-well plate.

    Article Title: Treating activated regulatory T cells with pramipexole protects human dopaminergic neurons from 6-OHDA-induced degeneration.
    Article Snippet: The cells were stimulated with anti- CD3/CD28 ImmunoCultTM Human CD3/ CD28 T Cell Activator (StemCell Technologies) for 4 days, and then treated for 24 h with pramipexole at concentrations of 2 and 200 ng/mL. .. Responder cells were labeled and isolated according to IL- 10 secretion using the IL- 10 secretion assay Cell Enrichment and Detection Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: Enhancement of the frequency and function of IL-10-secreting type I T regulatory cells after 1 year of cluster allergen-specific immunotherapy.
    Article Snippet: At the end of incubation, supernatants were collected from each culture and assayed for the presence of IL-10 by ELISA (R&D Systems, Minneapolis, Minn., USA). .. 10 7 PBMCs from each donor were stimulated for 16 h with 10 g/ml Der p 1 and IL-10-secreting Tr1 cells were isolated using the IL-10 Secretion Assay Cell Enrichment and Detection Kit (Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer’s protocol. .. Der p-specific IgE was measured using the UniCAP 100 system (Pharmacia Diagnostics, Uppsala, Sweden) and Der p-specific IgG4 using commercial ELISA kits according to the supplier’s instructions (ALK-Abello).

    Labeling:

    Article Title: Treating activated regulatory T cells with pramipexole protects human dopaminergic neurons from 6‐OHDA‐induced degeneration
    Article Snippet: The cells were stimulated with anti‐CD3/CD28 ImmunoCultTM Human CD3/CD28 T Cell Activator (StemCell Technologies) for 4 days, and then treated for 24 h with pramipexole at concentrations of 2 and 200 ng/mL. .. Responder cells were labeled and isolated according to IL‐10 secretion using the IL‐10 secretion assay Cell Enrichment and Detection Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: Treating activated regulatory T cells with pramipexole protects human dopaminergic neurons from 6-OHDA-induced degeneration.
    Article Snippet: The cells were stimulated with anti- CD3/CD28 ImmunoCultTM Human CD3/ CD28 T Cell Activator (StemCell Technologies) for 4 days, and then treated for 24 h with pramipexole at concentrations of 2 and 200 ng/mL. .. Responder cells were labeled and isolated according to IL- 10 secretion using the IL- 10 secretion assay Cell Enrichment and Detection Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: Cutting Edge: Inhibition of Glycogen Synthase Kinase 3 Activity Induces the Generation and Enhanced Suppressive Function of Human IL-10 + FOXP3 + –Induced Regulatory T Cells
    Article Snippet: .. Human iTregs were pretreated by GSK3 inhibitor (GSK3i) for 36 h and then stimulated with PMA and ionomycin for 5 h. IL-10–producing Tregs were then labeled using the IL-10 Secretion Assay-Cell Enrichment and Detection Kit (Miltenyi Biotec) according to the manufacturer’s instructions and then sorted into IL-10–producing and –nonproducing cells by FACS. ..

    FACS:

    Article Title: Cutting Edge: Inhibition of Glycogen Synthase Kinase 3 Activity Induces the Generation and Enhanced Suppressive Function of Human IL-10 + FOXP3 + –Induced Regulatory T Cells
    Article Snippet: .. Human iTregs were pretreated by GSK3 inhibitor (GSK3i) for 36 h and then stimulated with PMA and ionomycin for 5 h. IL-10–producing Tregs were then labeled using the IL-10 Secretion Assay-Cell Enrichment and Detection Kit (Miltenyi Biotec) according to the manufacturer’s instructions and then sorted into IL-10–producing and –nonproducing cells by FACS. ..

    Incubation:

    Article Title: Ci8 short, a novel LPS-induced peptide from the ascidian Ciona intestinalis, modulates responses of the human immune system.
    Article Snippet: The selective modulation of immunity is an emerging concept driven by the vast advances in our understanding of this crucial host defense system.. Invertebrates have raised researchers’ interest as potential sources of new bioactive molecules owing to their antibacterial, anticancer and immunomodulatory activities.. A LipoPolySaccharide (LPS) challenge in the ascidian Ciona intestinalis generates the transcript, Ci8 short, with cisregulatory elements in the 3′ UTR region that are essential for shaping innate immune responses.

    Selection:

    Article Title: Ci8 short, a novel LPS-induced peptide from the ascidian Ciona intestinalis, modulates responses of the human immune system.
    Article Snippet: The selective modulation of immunity is an emerging concept driven by the vast advances in our understanding of this crucial host defense system.. Invertebrates have raised researchers’ interest as potential sources of new bioactive molecules owing to their antibacterial, anticancer and immunomodulatory activities.. A LipoPolySaccharide (LPS) challenge in the ascidian Ciona intestinalis generates the transcript, Ci8 short, with cisregulatory elements in the 3′ UTR region that are essential for shaping innate immune responses.



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